Protective efficiency of an inactivated vaccine against Streptococcus iniae in olive flounder, Paralichthys olivaceus

  • Yong-Uk Jeong
  • Dharaneedharan Subramanian
  • Yeoung-Hwan Jang
  • Dong-Hwi Kim
  • So-Hyun Park
  • Kyung-il Park
  • Young-Don Lee
  • Moon-Soo Heo
Keywords: Streptococcus iniae, â-hemolytic streptococcosis, olive flounder, inactivated vaccine, relative percent survival


Streptococcus iniae is a causative agent of hemorrhagic septicemia in olive flounder, Paralichthys olivaceus, in Korea, resulting in serious economic losses. As a preventive measure, M VAC INIAE (Mastuken, Japan) was prepared from the S. iniae F2K strain and tested against the SI-36 strain prevalent on flounder fish farms on Jeju Island, Korea. F2K had a serotype of 38 (–) and SI-36 38 (+). The vaccine recognized both serotypes. It showed a very high effective immune response against S. iniae; the challenge test using the S. iniae SI-36 strain resulted in a relative percent survival (RPS) of 85.7-87.0% 2 weeks after vaccination and 71.0-80.0% 6 months after vaccination. Field vaccination and clinical challenge tests were performed at local Jeju aquafarms with S. iniae SI-36. These showed significantly reduced cumulative mortality when compared to the control group with RPS rates that ranged between 71-80%. Hence, the present study suggests that this vaccine showed a significant immune response against S. iniae and could be applied in commercial aquafarms as a therapeutic agent against â-hemolytic streptococcosis in cultured P. olivaceus.


Y.U. Jeong, D. Subramanian, D.H. Kim, S.H. Park, M.S. Heo [+]
Marine Pathogenic Microbes Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, South Korea
Y.-H. Jang
Jeju Special Self-Governing Province Ocean and Fisheries Research Institute, Pyoseon-myeon, Segwipo-si, Jeju 697-914, South Korea
K. Park
Department of Aquatic Life Medicine, Kunsan National University, Kunsan 573-701, South Korea
Y.D. Lee
Marine and Environmental Institute, Jeju National University, Jeju 690-814, South Korea
Y.U. Jeong and D. Subramanian contributed equally to this work.