In vitro antimicrobial activity of ethanolic extracts obtained from Ficus spp. leaves against the fish pathogen Aeromonas hydrophila

  • Halyna Tkachenko
  • Lyudmyla Buyun
  • Elżbieta Terech-Majewska
  • Zbigniew Osadowski
Keywords: antibacterial activity, Aeromonas hydrophila, infected rainbow trout, Ficus spp., ethanolic extracts, growth inhibition zone


The main goal of this study was to determine in vitro antimicrobial activity of ethanolic extracts obtained from the leaves of various Ficus species against Aeromonas hydrophila isolated locally from infected rainbow trout (Oncorhynchus mykiss Walbaum) with the aim of providing scientific rationale for the use of the plant in the treatment of bacterial infections induced by Aeromonas spp. in fish. Antimicrobial susceptibility testing was done on Muller-Hinton agar with the disc diffusion method. In the present study, most ethanolic extracts proved effective against the A. hydrophila tested, with 10-12 mm inhibition zones observed. A. hydrophila demonstrated the highest susceptibility to F. pumila. Among various species of Ficus with moderate activity against A. hydrophila, the highest antibacterial activities were noted for F. benghalensis, F. benjamina, F. deltoidea, F. hispida, and F. lyrata. Thus, Ficus can be used as a natural antiseptic and antimicrobial agent in veterinary practice. Further investigations need to be conducted to isolate and identify the bioactive compounds that can then be subjected to detailed pharmacological studies and the development of clinical applications. The alarming rate of increasing resistance in bacterial pathogens in aquaculture environments means that medicinal plants with antibacterial properties are very important as natural resources of new active compounds.


H. Tkachenko [+], Z. Osadowski
Institute of Biology and Environmental Protection
Pomeranian University in Slupsk, Poland
Arciszewskiego 22B, 76-200 Slupsk, Poland
L. Buyun
M. Gryshko National Botanical Garden, National Academy of Science of Ukraine, Kyiv, Ukraine
E. Terech-Majewska
Department of Epizootiology, University of Warmia and Mazury in Olsztyn, Poland