Effect of the herbicide Avans 330 SL on the liver pathomorphology of clinically healthy carp (Cyprinus carpio L.) and carp infected by Ichthyophthirius multifiliis

Abstract

This study was conducted on fingerlings of carp, Cyprinus carpio L., that were either clinically healthy or infected with a natural invasion of the ciliate, Ichthyophthirius multifiliis. The fish were exposed for 96 h to Avans 330 SL in a concentration of 2 mg trimethylosulfonium glyphosate l-1 water in order to establish its effect on the morphological pattern of the liver. It was demonstrated that the most frequent morphological deviations, particularly in fish infected with the protozoa and bathed in water with the addition of Avans, included regressive lesions, such as parenchymatous, vacuolar degeneration, and focal necrosis. Less often in these cases, and even less often in carp infected with I. multifiliis or only exposed to Avans, were disorders observed in the circulation in the form of hyperaemia and minor extravasations. Changes were noted in the ultrastructures of the mitochondria and the endoplasmic reticulum. In addition, the results obtained indicate that ciliate invasion intensified the occurrence of morphological lesions in the livers of carp that were exposed to Avans 330 SL in comparison to fish that were free of this infection.

CORRESPONDING AUTHOR:

Prof. dr hab. Józef Szarek, Uniwersytet Warmińsko-Mazurski, Wydział Medycyny Weterynaryjnej, Katedra Weterynaryjnej Ochrony Zdrowia Publicznego, ul. Oczapowskiego 13, 10-719 Olsztyn; Tel./Fax: +48 89 523 32 52;
e-mail: szarek@uwm.edu.pl