Verification of meiotic gynogenesis in brook trout, Salvelinus fontinalis (Mitchill) using microsatellite DNA markers

Abstract

The microsatellite DNA technique was applied to assess rapidly the overall success of treatments designed to induce gynogenesis. The results of gynogenesis were verified based on the analysis of two microsatellite loci (Sfo-296 and Sfo-262). Three brook trout, Salvelinus fontinalis (Mitchill), females, one brown trout, Salmo trutta morpha fario L., male, and their offspring (30 individuals from each female) were studied. Locus Sfo-292 was monomorphic in the studied brook trout offspring while Sfo-262 was polymorphic. The paternal genotype was absent in the studied gynogenotes. This simple microsatellite-based technique could be generally applicable for the verification of gynogenesis in brook trout when the UV-irradiated milt of brown trout is used.

CORRESPONDING AUTHOR:

Dorota Fopp-Bayat, Uniwersytet Warmińsko-Mazurski, Wydział Ochrony Środowiska i Rybactwa, Katedra Ichtiologii, ul. Oczapowskiego 5, 10-718 Olsztyn; Tel./Fax: +48 89 5234772, +48 89 5233754, e-mail: foppik@uwm.edu.pl