Androgenesis of ide, Leuciscus idus (L.), using chub, Leuciscus cephalus (L.), eggs     [SHORT COMMUNICATION]

Dariusz Kucharczyk; Katarzyna Targońska; Marek J. Łuczyński; Andrzej Szczerbowski; Maciej Kwiatkowski; Roman Kujawa

Dariusz Kucharczyk
Katarzyna Targońska
Marek J. Łuczyński
Andrzej Szczerbowski
Maciej Kwiatkowski
Roman Kujawa

Keywords: INTERSPECIFIC ANDROGENOTES, GENOME MANIPULATION, COLOR MARKER, CYPRINIDS

Abstract

The focus of this study was the agdrogenesis of ide, Leuciscus idus (L.), using eggs from other species. Chub, Leuciscus cephalus (L.), oocytes were genetically inactivated using UV irradiation. The eggs used in the experiment were obtained from dark-colored chub females, whereas milt was taken from yellow-colored (recessive marker) ide males. The highest yield of ide androgenesis was noted when eggs were exposed to a thermal shock 3 hrs 00 min after activation. Androgenetic origin (haploid or diploid embryos) was checked using a recessive color marker (“blond”).

CORRESPONDING AUTHOR:

Dariusz Kucharczyk, University of Warmia and Mazury, Department of Lake and River Fisheries, Oczapowskiego 5, 10-957 Olsztyn, Poland, Tel./Fax: +48 895234215, +48 895233969; e-mail: darekk@uwm.edu.pl