Spawning induction in kutum, Rutilus frisii kutum (Kamensky), with different hormones: Analysis of hormone profiles and induced spawning success         [RESEARCH ARTICLE]

Bahram Falahatkar; Samaneh Poursaeid; Hadi Ershad Langroudi; Iraj Efatpanah; Bahman Meknatkhah; Mehdi Rahmati

doi:10.2478/aopf-2013-0028

Bahram Falahatkar
Samaneh Poursaeid
Hadi Ershad Langroudi
Iraj Efatpanah
Bahman Meknatkhah
Mehdi Rahmati

DOI: https://doi.org/10.2478/aopf-2013-0028

Keywords: Kutum, spawning induction, common carp pituitary, human chorionic gonadotropin, GnRH analogues, domperidone, sex steroids

Abstract

The objective of this study was to evaluate the effectiveness of different hormones on spawning success and sex steroid changes in Caspian Kutum, Rutilus frisii kutum. Groups of five fish were injected intramuscularly as follows: 2 mg kg^-1 common carp pituitary extract (CPE) as the positive control, 100 IU kg^-1 human chorionic gonadotropin (hCG), 2 µg kg-1 luteinizing hormone releasing hormone analog (LHRHa2), 10 µg kg-1 synthetic analogue gonadoliberins (sGnRHa), 0.2 mL kg-1 Ovaprim which is a complex of salmon GnRH analogue with a dopamine receptor antagonist (domperidone), a combination of 2 µg kg-1 LHRHa2 and 100 IU kg^-1 hCG, a complex of 10 µg kg^-1 sGnRHa and 100 hCG IU kg^-1, and saline solution as the negative control. Blood samples were taken simultaneously with injections and after ovulation. The results showed that no fish ovulated in the negative control group. In the CPE treatment group, 4 of 5 fish ovulated. In the other experimental groups; no response was observed in the hCG group, while the females injected with sGnRHa-alone, LHRHa2-alone, and sGnRH+hCG had low spawning percentages. Of the hormones tested, the combination of sGnRHa+domperidone was the most effective spawning inducer and the greatest spawning success was obtained. Significant decreases after injection were observed in plasma concentrations of 17â estradiol, 17á-hydroxy progesterone, and progesterone in all treatments, while plasma testosterone concentrations increased significantly. This study revealed that a combined sGnRHa and dopamine antagonist can improve the effectiveness of artificial reproduction of Kutum. Since the combination of sGnRHa and domperidone is purer than CPE and has the most effective in inducing ovulation, it is preferred for inducing spawning in this species.

CORRESPONDING AUTHOR:

B. Falahatkar [+],
Fisheries Department, Faculty of Natural Resources
University of Guilan
Sowmeh Sara, P.O. Box 1144, Guilan, Iran
Tel./Fax: +98-182-322 3599; +98-182-322 3600
e-mail: falahatkar@guilan.ac.ir
S. Poursaeid
Young Researchers Club and Elite, Rasht Branch
Islamic Azad University, Rasht, Iran
H.E. Langroudi
Fisheries Department, Faculty of Natural Resources
Islamic Azad University, Lahijan Branch, Lahijan, Guilan, Iran
I. Efatpanah, B. Meknatkhah, M. Rahmati
Dr. Yousefpour Fish Hatchery Center, Siahkal, Guilan, Iran