PROPERTIES AND CRYOPRESERVATION OF DANUBE SALMON (Hucho hucho) MILT

Jan Glogowski; Igor Babiak; Krzysztof Goryczko; Stefan Dobosz; Henryk Kuźmiński

Jan Glogowski
Igor Babiak
Krzysztof Goryczko
Stefan Dobosz
Henryk Kuźmiński

Keywords: CRYOPRESERVATION, DANUBE SALMON, FERTILIZATION, MOTILITY, SPERM

Abstract

Motility and concentration of spermatozoa, as well as osmolality, protein content, activity of aspartate aminotransferase (AspAT), acid (AcP) and alkaline (AP) phosphatase of seminal plasma were determined in 8 samples of Danube salmon (Hucho hucho) fresh milt. Semen pooled from 6 males was cryopreserved in 8 extenders containing DMSO or glycerol as cryoprotectants. Extender containing 0.3 M glucose + 25 mM KCl + 10% egg yolk + 10% DMSO was the most suitable (87.5% of eyed eggs, as compared to control fertilization). Percentage of eyed eggs correlated with post-thaw spermatozoa motility.