Molecular detection of Renibacterium salmoninarum in rainbow trout (Oncorhynchus mykiss) from Poland
Keywords:
Renibacterium salmoninarum, fish, bacterial kidney disease, infection
Abstract
Renibacterium salmoninarum causes bacterial kidney disease mainly in salmonid fish. Oligonucleotide primers incorporating R. salmoninarum unique sequences were designed to amplify a 501 bp region of the gene encoding a 57 kDa soluble extra-cellular protein. The primers did not amplify other wide varieties of aquatic or piscine bacteria Aeromonas salmonicida or Yersinia ruckeri. This assay provides a molecular description and definitive identification of R. salmoninarum in Poland.